Isolation of Impurities
Laboratorio Cifga S.A. can help you to purify your compound or impurity from complex mixtures as well as in the characterization of any chemical available in sufficient amount.
Isolation of suitable quantities –often up to milligrams level- is required to perform characterization.
Semi-preparative or preparative chromatography methods can be developed to isolate impurities or chemicals that cannot be achieved by other purification methods such as crystallization, or whose synthesis consisting in too many steps with low recoveries. In addition, bulk purification can be previously accomplished by Liquid-liquid extraction, purification on several open column types: silica gel (normal phase), C18 and C8 (reverse phase), Hypercarb for very polar molecules, size exclusion, ion exchange ….
Isolation of chemical compounds by preparative chromatography allows to achieve a pure standard of any unknown impurity besides structural elucidation and quantification can be perform by nuclear magnetic resonance and mass spectrometry means.
EQUIPMENTS
Our equipment allows us to achieve the necessary analyses for the determination of the structure of an impurity or pure compounds: NMR analyses (1H, 13C, DEPT, COSY, HMBC, HSQC…), UPLC/MS/MS, HPLC-UV
NMR Varian 500 MHz and NMR Bruker 750 MHz for NMR analyses, 1H, 13C, DEPT, COSY, HMBC, HSQC…
Preparative HPLC system of Waters. Consists of an autosampler and fraction collector coupled to the Waters 3100 single quadrupole mass detector.
UPLC Waters Acquity equipped with a triple quadrupole Quattro Premier XEVO detector.
HPLC Waters Alliance equipped with a Waters dual absorbance detector and a Waters Micromass QMicro detector.
Laboratorio Cifga can help you to isolate and characterize your chemicals thanks to more than 15 years of expertise.
Moreover, a Certificate of Analysis (CoA) can be issued according to the product characterization.
